The Prevalence of Blastocystis hominis in Wadi Al-Shati Province, Libya

The present work was planned to estimate the prevalence of Blastocystis hominis among random population of Wadi Al-Shati province. Stool specimens from 1000 randomized individuals were collected (during the period from the first of August 2007 to end of March 2008) from seventeen rural localities of Wadi Al-Shati province, presenting different sexes and ages (427 males and 573 females and aged from 1 to 90 years). Stool samples were examined using direct wet mounts and concentration method. The overall prevalence of B. hominis was 21.20% (212/1000), the prevalence rates varied from 12.50 to 38.23% among random population of different localities of Wadi Al-Shati province. The highest positivity was found in Mansurah locality (38.23%) followed by Quttah locality (31.25%. Out of 15 samples examined in Ashkeda, none was found positive for B. hominis. Prevalence rates between males and females were 22.24% (95/427) and 20.41% (117/573) respectively. The difference was not statistically significant (P>0.05). The age group 11 to 20 years showed the highest rate of infection (24.00 %), and the lowest (13.77 %) was in 1 to 10 years. Fecal specimens were examined by three direct wet mounts samples (normal saline, iodine and eosin stains) and formalin-ether concentration sedimentation smears in iodine stains. 183 samples (18.30%) were found to be positive for B. hominis in direct smears in normal saline, 197 samples (19.70%) were found to be positive in direct smears in iodine and 196 samples (19.60%) were found to be positive in direct smears in eosin stain for B. hominis. Concentration sedimentation detected B. hominis in 212 samples (21.20%). Comparative analysis of the results, showed no significant difference of sensitivity (P 0.05) between direct smears and formalin-ether sedimentation for the diagnosis of B. hominis in fresh fecal material.