Mohamed M. Ahmed, Abdalgader Saleh Ali, Mahtab Alam Faraj Omar Matoug2018-02-192018-02-192011https://repository.sebhau.edu.ly/handle/1/601Introduction: Transaminases are enzymes involved in transfer of amino groups from α-amino acid to α-keto acid using pyridoxal phosphate as a cofactor. Aspartate aminotransferase (AST) is widely distributed, whereas, alanine aminotransferase (ALT) is high in liver and to a lesser extent in skeletal muscle, kidney and heart. Both rise in serum after tissue damage. Bilirubin is an end product of the breakdown of haem, and its deposition leads to hyperbilirubinaemia which can cause brain damage, as a result long-life neurological disability. Objective: This study was carried-out to evaluate the ALT and AST activity, and the bilirubin (total and direct) level in newborn Libyan babies, as well as evaluating their inter-relationship. Materials and Methods: Blood was collected for routine investigation from 200 newborn Libyan babies, aged 1 to 18 days (X = 6.3 ± 4.0 days), admitted in the neonatal unit, Sebha Teaching Hospital, Sebha, Libya. A part of this blood were used to investigate the ALT and AST activity, and the bilirubin level, which were performed by Kinetic test-IFCC recommended from Biomaghreb (Tunisa) and Biocon (Hecke, Germany) based on Jendrassik-Grof method, respectively. Results: In these two hundred newborn babies, the activity of ALT was 23.59 ± 13.5 U/l and AST was 58.20 ± 35.6 U/l. We observed a significant difference between the two means (P<0.001), as well as a significant correlation (r = 0.75, P< 0.001). The ratio of ALT to AST was 1: 2.47. The total and direct bilirubin was 9.5 ± 4.9 mg/dl and 1.3 ± 1.5 mg/dl, respectively. We found no significant correlation between the activity of AST and ALT, and the bilirubin level. However, in group-I (41.5%) of these babies (n = 83, age = 5.9 ± 3.5 days) the total bilirubin was 9.2 ± 5.3 mg/dl and the direct bilirubin was 0.7 ± 0.5 mg/dl. While, the ALT activity was 19.1 ± 9.7 U/l and AST activity was 46.1 ± 17.2 U/l. The ratio of ALT to AST was 1 : 2.41. Again, we found , a significant difference between the means of AST and ALT activity (P < 0.001), as well as a significant correlation (r = 0.44, P < 0.001). In the other group-II, (58.5%, n = 117) the total bilirubin was 9.6 ± 4.7 mg/dl, but the direct bilirubin was 3.0 ± 2 mg/dl. While, the activity of ALT was 25.7 ± 15.7 U/l and AST was 65.9 ± 43.2 U/l. The ratio of ALT to AST was 1: 2.56. Interestingly, we observed a significant correlation between the activity of AST and ALT (r = 0.78, P < 0.001), and negative correlation between the activity of both AST and ALT, and the bilirubin (P = NS). Conclusion: Establishment of reference values for newborn babies would be helpful in achieving the most accurate diagnosis.enAlanine aminotransferase, Aspartate aminotransferase, Bilirubin, Newborn babies.Article