Detection of bacterial contamination in adult and infant powdered milk consumed in Khartoum State
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Date
2014
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Publisher
Sebha University
Abstract
This study was carried out to evaluate the microbiological contamination of powdered milk, The prevalence of pathogenic bacteria and antimicrobial susceptibility patterns, in a total of 60 samples of commercial dried milk products (adult powder milk and infant powdered milk 30 samples each) used by consumers in Khartoum state (Sudan), conventional culture method were used for isolation and identification.
The total viable bacterial count of adults powdered milk ranged between 1-38 (CFU)/ml, and the total viable bacterial count for infants ranging from 0-28 (CFU)/ ml. The total number of different species of bacteria isolated from adult powdered milk samples was 45; Bacillus cereus isolates were 17 (37% ), Bacillus leichinformis were 11 (24% ), Bacillus mycoides were 3 (7%). Staphylococcus aureus 9 (20%), Listeria monocytogenes 5 (11%). The total number of different species of bacteria isolated from infant formula samples was 39; Bacillus cereus isolates were 12 (30%), Bacillus leichinformis 7 (18%), Bacillus mycoides 2 about (5%). Staphylococcus aureus 2 (5%), Listeria monocytogenes 3 (7.9%), Enterobacter sakazakii 9 (23%), Escherichia coli 4 (9%). In relation to antibiotic resistance of bacteria isolated from both infant formula and adult’s powdered milk. Staphylococcus aureus resistance against Amoxycillin and Tetracycline was between (45-60%), Erythromycin (30-40%), Ceftriaxone Ciprofloxacin (25-30%) and Cefixime was (20-35%). Bacillus species resistance against tetracycline was (30-50%) , against Amoxycillin, Ceftriaxone, Ciprofloxacin was (20-30%) and Cefixime was (20-35%). Listeria monocytogenes resistance against tetracycline was (40%), Amoxycillin (25-30%), Ciprofloxacin (12-25%), Cefixime (20%), Ceftriaxone (15-20%), Erythromycin (10-20%). Enterobacter sakazaki resistance against Amoxycillin was (50%), tetracycline (40%), Ciprofloxacin, Cefixime, Ceftriaxone (15-20%). The lowest resistance of all isolates was seen against Amikacin and Meropenem (0-5%).